When applying non‐sizzling‐start Taq polymerase, it is best to transfer the reactions instantly from ice to some PCR block pre‐heated to fifty°C, without letting them to sit at area temperature. In particular, we found the SARS‐CoV‐2 N1 primer established was hugely delicate to place temperature incubation, while N2 reactions could https://www.pinterest.com/pin/993958580252567964/